曾偉基博士
副教授
副教授
TEACHING AREAS
Journey to the Exotic World of Microorganisms
QUALIFICATIONS
1995 | BSc in Biochemistry, The Chinese University of Hong Kong |
1999 | PhD, The Chinese University of Hong Kong |
EMPLOYMENT
2017 - now | Associate Professor, School of General Education and Languages, Technological and Higher Education Institute of Hong Kong |
2014 - 2017 | Assistant Professor, School of General Education and Languages, Technological and Higher Education Institute of Hong Kong |
2009 - 2014 | Research Assistant Professor, Oral BioSciences, Faculty of Dentistry, The University of Hong Kong |
2004 - 2009 | Research Assistant Professor, Department of Biochemistry, The Chinese University of Hong Kong |
2004 - 2004 |
Chief Scientist, GeneHarbor (Hong Kong) Biotechnologies Limited |
2002 - 2004 |
Scientific Officer, Department of Biochemistry, The Chinese University of Hong Kong |
2000 - 2002 |
Postdoctoral Fellow, Department of Biochemistry, The Chinese University of Hong Kong |
HONORARY APPOINTMENT
2011 - 2017 | Honorary Assistant Professor, Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong |
AWARDS
RESEARCH INTERESTS
PROFESSIONAL SOCIETIES
TEACHING DEVELOPMENT GRANTS
2008 -2010 |
Disciplinary Co-ordinator. Title: The development of student-oriented teaching and learning resources in science and a science enrichment program for secondary 3-4 students. Quality Education Fund, HKSAR(HK$3,500,000) |
2006 -2008 |
Disciplinary Co-ordinator. Title: A science enrichment program for secondary 3-4 students Quality Education Fund, HKSAR(HK$2,000,000) |
2005 -2008 |
Co-supervisor. Title: Engaging science students in the design and enactment of assessment Teaching Development Grant, The Chinese University of Hong Kong(HK$200,000) |
EXTERNAL RESEARCH GRANTS
2017 -2018 |
Principal Investigator. Title: Made-to-order: engineering and optimization of “tunable” yeasts for phytate hydrolysis Faculty Development Scheme, University Grants Council, HKSAR(HK$754,500) |
2016 |
Principal Investigator. Title: Working hand-in-hand: building engineered yeasts for semi-synthetic cephalosporins Faculty Development Scheme, University Grants Council, HKSAR(HK$725,648) |
2015 -2017 |
Principal Investigator. Title: A sticky problem: functional analysis of three alcohol dehydrogenases involved in biofilm matrix production in the emerging human fungal pathogen Candida dubliniensis Health and Medical Research Fund, Food and Health Bureau, HKSAR(HK$823,440) |
2013 -2015 |
Principal Investigator. Title: Perturbation of pH homeostasis: a comparative study on the inhibitory effect of purpurin on membrane-bound ATPases in Candida albicans Health and Medical Research Fund, Food and Health Bureau, HKSAR(HK$743,520) |
2013 -2014 |
Co-Investigator. Title: Anti-infective therapy of Er,Cr:YSGG laser on contaminated zirconia implant surfaces Health and Medical Research Fund, Food and Health Bureau, HKSAR(HK$78,000) |
2012 -2014 |
Principal Investigator. Title: Investigation of the functional significance of phytase activity in Candida albicans: gene disruption, pH regulation, and virulence study Health and Medical Research Fund, Food and Health Bureau, HKSAR(HK$704,448) |
2011 -2012 |
Principal Investigator. Title: From vital to lethal: functional analysis of apoptosis-inducing factor in the human fungal pathogen Candida albicans Research Fund for the Control of Infectious Diseases, Food and Health Bureau, HKSAR(HK$80,000) |
2009 -2010 |
Principal Investigator. Title: Generation of focused libraries by combinatorial biocatalysis with anti-Candidal activity: synthesis and in vitro study Research Fund for the Control of Infectious Diseases, Food and Health Bureau, HKSAR(HK$704,448) |
2009 -2011 |
Co-Investigator. Title: Investigating the involvement of PI3K/PDK-1/Akt pathways and Notch signals in SARS-CoV membrane-induced apoptosis Research Fund for the Control of Infectious Diseases, Food and Health Bureau, HKSAR(HK$741,448) |
2008 -2010 |
Principal Investigator. Title: Characterization of highly active, thermostable mutants of Trigonopsis variabilis D-amino acid oxidase for antibiotics production: kinetic measurements, structural studies and beyond General Research Fund, Research Fund Council, HKSAR(HK$799,000) |
2001 -2003 |
Co-Investigator. Title: Efficient, economical and environmentally-friendly production of key intermediates of major antibiotics Innovation and Technology Fund, Innovation and Technology Council, HKSAR(HK$2,463,480) |
SELECTED PUBLICATIONS
Selected Journal Articles (2009 onwards)
Yang, H.P., Tsang, P.C.S., Lam, O.L.T. and Tsang, W.K. (2019) Candida albicans SAP9: a novel hyphae formation factor. (Submitted)
Yang, H.P., Tsang, P.C.S., Lam, O.L.T. and Tsang, W.K. (2019) Candida albicans Sap7 mediates epithelial cell invasion and virulence. (Submitted)
Lam O.L.T. and Tsang, W.K. (2018) Antifungal activity of commercial mouthrinses against Candida albicans. Dent Open J, 5:11-18.
Tsang, W.K., Fong, W.P. and Samaranayake, L.P. (2017) Candida albicans orf19.3727 encodes phytase activity and is essential for human tissue damage. PLoS ONE, 12:e0189219.
Tsang, W.K. and Lam, O.L.T. (2014) SEM image of Candida albicans biofilms on plastic coupons. Austin Journal of Clinical Case Reports, 1:1011.
Yang, H.P., Tsang, P.C.S. and Tsang, W.K. (2014) Melatonin inhibits biofilm formation in Candida parapsilosis. Journal de Mycologie Médicale, 24, 360-361.
CONFERENCE PRESENTATIONS
Tsang, W.K. (2019) “A Green Journey to the Making of Magic Bullets”. In International Conference on Applied Education, Technology and Innovation (THEi AETI 2019), Hong Kong. (Oral Presentation)
Tsang, W.K., Fong, W.P. and Samaranayake, L.P. (2018) “Overexpression of Ifd6p, an aryl alcohol dehydrogenase, reduced biofilm matrix biomass and increased susceptibility to stressors in Candida dubliniensis biofilms”. In The 66th Annual Meeting of Japanese Association of Dental Research, Hokkaido, Japan.
Tsang, W.K. and Fong, W.P. (2018) “Working hand-in-hand: building engineered yeast for semi-synthetic cephalosporins”. In THEi 2018 Research Symposium, Hong Kong.
Tsang, W.K., Fong, W.P. and Samaranayake, L. P. (2018) “Characterisation of Candida albicans orf19.3727 in fungal physiology and pathobiology”. In THEi 2018 Research Symposium, Hong Kong.
Xie, Z.M., Yeung, C.H.T., Fong, W.P. and Tsang, W.K. (2018) “High Level Expression of Secretory Phytases in Pichia pastoris”. In 9th International Congress on Biocatalysis (biocat2018), Hamburg, Germany.
Tsang, W.K. and Yiu, J.W.K. (2018) “Support Services for Students with Special Educational Needs (SEN) in General Education Curriculum”. In Federation for Self-financing Tertiary Education (FSTE) Conference 2018: Striving for Quality Education, Hong Kong. (Oral Presentation)
Tsang, W.K. (2017) “Inclusion of Experiential Learning Activity for Teaching and Learning of Scientific Thinking”. In Federation for Self-financing Tertiary Education (FSTE) Conference 2017: Innovative Learning and Teaching, Hong Kong. (Oral Presentation)
Tsang, W.K., Lam, O.L.T., Fong, W.P. and Samaranayake, L.P. (2017) “Purpurin, an anthraquinone in madder root, interferes with pH homeostasis in Candida albicans via specific inhibition of plasma membrane ATPase Pma1p”. In The 65th Annual Meeting of Japanese Association of Dental Research, Tokyo, Japan.
Chan, R.S., McGrath, C., Tsang, W.K. and Lam, O.L.T. (2017) “Effectiveness of an ultrasonic denture hygiene intervention program among community-dwelling elders”. In The 65th Annual Meeting of Japanese Association of Dental Research, Tokyo, Japan.
Tsang, W.K. and Lam, O.L.T. (2016) “Solasodine inhibits Candida albicans adhesion at sub-MIC level”. In The 94th General Session & Exhibition of the International Association of Dental Research, Seoul, Republic of Korea.
Lam, O.L.T., Cheung, B.P.K. and Tsang, W.K. (2016) “Anti-biofilm activity of commercial toothpastes against Staphylococcus aureus.” In The 94th General Session & Exhibition of the International Association of Dental Research, Seoul, Republic of Korea.
Tsang, W.K., Fong, W.P. and Samaranayake, L.P. (2015) “Deletion of Candida albicans PHO112 reduces phytase activity, hyphal development, and attenuates virulence.” In The 63rd Annual Meeting of Japanese Association of Dental Research, Fukuoka, Japan.
Wong, K.S, Fong, W.P. and Tsang, W.K. (2015) “Effects of an extra Trp113Tyr substitution on yeast D-amino acid oxidase variant.” In Practical Social and Industrial Symposium 2015, Vocational Training Council, Hong Kong.
Yang, H.P., Tsang, P.C.S., Pow, E.H.N., Lam, O.L.T. and Tsang, W.K. (2015) “Candida albicans SAP9: a gene which novel function in hyphal development in Candida albicans.” In The 29th Annual Scientific Meeting International Association for Dental Research, Bali, Indonesia.
Ng, Y.C., Lai, C.F., Cheung, B.P.K., McGrath, C., Tsang, W.K. and Lam, O.L.T. (2015) “Mouthrinse effectiveness against Candida glabrata.” In The 29th Annual Scientific Meeting International Association for Dental Research, Bali, Indonesia.
Tsang, W.K., Chau, D.K.Y. and Yang, H.P. (2015) “Anisomycin halts Candida albicans morphogenesis.” In The 19th Congress of the International Society for Human and Animal Mycology, Melbourne, Australia.
Tsang, W.K. and Fong, W.P. (2014) “Functional characterization of Candida albicans apoptosis-inducing factor.” In Health Research Symposium 2014: Translating Health Research into Policy and Practice for Health of the Population, Food and Health Bureau, Hong Kong.
PATENTS
Wang, J., Tsang, W.K., Yap, H.K., Chen, J.M, Siu, Y.L.P. and Tsang, S.Y. (2010) Two-step enzyme method for preparing 7-aminocephalosporanic acid. US Patent 2010/112633 A1.
Wang, J., Yap, H.K., Tsang, W.K., Lui, Y.S.R., Siu, Y.L.P., Tsang, S.Y. and Yau, M.H. (2008) Recombinant D-amino acid oxidase. US Patent 2008/0009052 A1.
Wang, J., Tsang, W.K., Yap, H.K., Liu, Z.M., Siu, Y.L.P. and Tsang, S.Y. (2008) Escherichia coli expression vector and the uses thereof. European Patent Application EP1925670 A1.
Wang, J., Yap, H.K., Tsang, W.K., Lui, Y.S.R., Siu, Y.L.P., Tsang, S.Y. and Yau, M.H. (2007) The use of recombinant D-amino acid oxidase. WO 2007051355 A1.